Ziege anti-Kaninchen IgG Antikörper (DyLight 680)

Produktnummer ABIN6699112

Hersteller Produkt- Nr.: 611-144-002

Kurzübersicht für Ziege anti-Kaninchen IgG Antikörper (DyLight 680) (ABIN6699112)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 680

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Kaninchen

Wirt: Ziege

Hersteller Produkt- Nr.: 611-144-002

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody DyLight™ 680 Conjugated

Produktmerkmale: Goat anti-Rabbit IgG Antibody DyLight™680 Conjugation, Goat anti-Rabbit IgG DyLight™ 680 Conjugated Antibody,Anti-Rabbit IgG Antibody DyLight™680 generated in goat detects rabbit IgG.

Aufreinigung:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. This antibody will react with heavy chains of Rabbit IgG and with light chains of most Rabbit immunoglobulins.

Immunogen: Rabbit IgG whole molecule

Labeling Ratio: 2.8

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Rabbit IgG Antibody DyLight™680 has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, IP

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Li, Krafft, Zeng, Duan, Qi, Shao, Xue, Zhang: "Microglia Autophagy Mediated by TMEM166 Promotes Ischemic Stroke Secondary to Carotid Artery Stenosis." in: Aging and disease, Vol. 15, Issue 3, pp. 1416-1431, (2024) (PubMed).

Szewczyk, Günther, Japtok, Frech, Naumann, Lee, Hermann: "FUS ALS neurons activate major stress pathways and reduce translation as an early protective mechanism against neurodegeneration." in: Cell reports, Vol. 42, Issue 2, pp. 112025, (2023) (PubMed).

Cantacorps, Coull, Falck, Ritter, Lippert: "Gut-derived peptide hormone receptor expression in the developing mouse hypothalamus." in: PloS one, Vol. 18, Issue 8, pp. e0290043, (2023) (PubMed).

Cuevas-Navarro, Wagner, Van, Swain, Mo, Columbus, Allison, Cheng, Messing, Turbyville, Simanshu, Sale, McCormick, Stephen, Castel: "RAS-dependent RAF-MAPK hyperactivation by pathogenic RIT1 is a therapeutic target in Noonan syndrome-associated cardiac hypertrophy." in: Science advances, Vol. 9, Issue 28, pp. eadf4766, (2023) (PubMed).

Lopez, Bonsor, Sale, Urisman, Mehalko, Cabanski-Dunning, Castel, Simanshu, McCormick: "The ribosomal S6 kinase 2 (RSK2)-SPRED2 complex regulates the phosphorylation of RSK substrates and MAPK signaling." in: The Journal of biological chemistry, Vol. 299, Issue 6, pp. 104789, (2023) (PubMed).

García-Baos, Gallego-Landin, Ferreres-Álvarez, Puig-Reyne, Castro-Zavala, Valverde, Martín-Sánchez: "Effects of fast-acting antidepressant drugs on a postpartum depression mice model." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 154, pp. 113598, (2022) (PubMed).

Tao, Zhu, Diaz-Perez, Yu, Foley, Stewart, Casero, Steet, Zhai: "Phenylbutyrate modulates polyamine acetylase and ameliorates Snyder-Robinson syndrome in a Drosophila model and patient cells." in: JCI insight, Vol. 7, Issue 13, (2022) (PubMed).

Orengo, Nitschke, van der Heijden, Ciaburri, Orr, Zoghbi: "Reduction of mutant ATXN1 rescues premature death in a conditional SCA1 mouse model." in: JCI insight, Vol. 7, Issue 8, (2022) (PubMed).

Alegre-Zurano, Berbegal-Sáez, Luján, Cantacorps, Martín-Sánchez, García-Baos, Valverde: "Cannabidiol decreases motivation for cocaine in a behavioral economics paradigm but does not prevent incubation of craving in mice." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 148, pp. 112708, (2022) (PubMed).

Cuevas-Navarro, Van, Cheng, Urisman, Castel, McCormick: "The RAS GTPase RIT1 compromises mitotic fidelity through spindle assembly checkpoint suppression." in: Current biology : CB, Vol. 31, Issue 17, pp. 3915-3924.e9, (2022) (PubMed).

Castro-Zavala, Martín-Sánchez, Montalvo-Martínez, Camacho-Morales, Valverde: "Cocaine-seeking behaviour is differentially expressed in male and female mice exposed to maternal separation and is associated with alterations in AMPA receptors subunits in the medial prefrontal cortex." in: Progress in neuro-psychopharmacology & biological psychiatry, Vol. 109, pp. 110262, (2022) (PubMed).

Doerfler, Park, Assini, Youssef, Saxena, Yaseen, De Giorgi, Chuecos, Hurley, Li, Marcovina, Bao, Boffa, Koschinsky, Lagor: "LPA disruption with AAV-CRISPR potently lowers plasma apo(a) in transgenic mouse model: A proof-of-concept study." in: Molecular therapy. Methods & clinical development, Vol. 27, pp. 337-351, (2022) (PubMed).

García-Baos, Puig-Reyne, García-Algar, Valverde: "Cannabidiol attenuates cognitive deficits and neuroinflammation induced by early alcohol exposure in a mice model." in: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Vol. 141, pp. 111813, (2021) (PubMed).

Navarro, Tapia-Galisteo, Martín-García, Tarín, Corbacho, Gómez-López, Sánchez-Tirado, Campuzano, González-Cortés, Yáñez-Sedeño, Compte, Álvarez-Vallina, Sanz: "TGF-β-induced IGFBP-3 is a key paracrine factor from activated pericytes that promotes colorectal cancer cell migration and invasion." in: Molecular oncology, Vol. 14, Issue 10, pp. 2609-2628, (2021) (PubMed).

Cantacorps, Montagud-Romero, Luján, Valverde: "Prenatal and postnatal alcohol exposure increases vulnerability to cocaine addiction in adult mice." in: British journal of pharmacology, Vol. 177, Issue 5, pp. 1090-1105, (2021) (PubMed).

Nitschke, Coffin, Xhako, El-Najjar, Orengo, Alcala, Dai, Wan, Liu, Orr, Zoghbi: "Modulation of ATXN1 S776 phosphorylation reveals the importance of allele-specific targeting in SCA1." in: JCI insight, Vol. 6, Issue 3, (2021) (PubMed).

Li, Gu, Zhang, Yu, Feng, Lou, Lv, Chen: "RNF115 deletion inhibits autophagosome maturation and growth of gastric cancer." in: Cell death & disease, Vol. 11, Issue 9, pp. 810, (2021) (PubMed).

Ma, Zhu, Lu, Xie, Li, Shin, Qiang, Liu, Dou, Xiao, Wang, Jia, Long, Yang, Fang, Jiang, Zhang, Zhang, Zhai, Liu, Li: "Nicotinamide mononucleotide adenylyltransferase uses its NAD+ substrate-binding site to chaperone phosphorylated Tau." in: eLife, Vol. 9, (2021) (PubMed).

De Giorgi, Li, Hurley, Barzi, Doerfler, Cherayil, Smith, Brown, Lin, Bissig, Bao, Lagor: "Targeting the Apoa1 locus for liver-directed gene therapy." in: Molecular therapy. Methods & clinical development, Vol. 21, pp. 656-669, (2021) (PubMed).

Alegre-Zurano, Martín-Sánchez, Valverde: "Behavioural and molecular effects of cannabidiolic acid in mice." in: Life sciences, Vol. 259, pp. 118271, (2020) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to DyLight™680.