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Esel anti-Kaninchen IgG Antikörper (DyLight 488) - Preadsorbed

WB, FLISA, FM, DB Polyclonal IgG DyLight 488
Rockland
Produktnummer ABIN6699101
Hersteller Produkt- Nr.: 611-741-127

Kurzübersicht für Esel anti-Kaninchen IgG Antikörper (DyLight 488) - Preadsorbed (ABIN6699101)

Target

IgG

Klonalität

  • 3453
  • 231
  • 16
  • 1
Polyklonal

Konjugat

  • 666
  • 423
  • 422
  • 400
  • 274
  • 262
  • 235
  • 118
  • 105
  • 72
  • 67
  • 64
  • 61
  • 58
  • 58
  • 50
  • 44
  • 43
  • 40
  • 40
  • 39
  • 39
  • 36
  • 36
  • 34
  • 32
  • 31
  • 30
  • 29
  • 29
  • 29
  • 29
  • 29
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 28
  • 27
  • 26
  • 26
  • 25
  • 21
  • 21
  • 19
  • 17
  • 17
  • 16
DyLight 488

Applikation

  • 2120
  • 2053
  • 1900
  • 1873
  • 999
  • 919
  • 895
  • 520
  • 423
  • 234
  • 234
  • 149
  • 102
  • 98
  • 90
  • 84
  • 51
  • 33
  • 30
  • 28
  • 25
  • 20
  • 15
  • 11
  • 9
  • 8
  • 7
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp

IgG
  • Reaktivität

    • 752
    • 706
    • 608
    • 474
    • 410
    • 244
    • 213
    • 176
    • 146
    • 141
    • 124
    • 110
    • 107
    • 101
    • 83
    • 62
    • 48
    • 33
    • 28
    • 16
    • 15
    • 10
    • 8
    • 5
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Kaninchen

    Wirt

    • 1787
    • 1583
    • 568
    • 284
    • 122
    • 112
    • 41
    • 32
    • 22
    • 21
    • 15
    • 11
    • 11
    Esel

    Hersteller Produkt- Nr.

    611-741-127

    Hersteller

    Rockland

    Verwendungszweck

    Rabbit IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

    Kreuzreaktivität (Details)

    Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

    Produktmerkmale

    Donkey Anti-Rabbit IgG Antibody DyLight 488™ Conjugated, Donkey Anti Rabbit IgG DyLight 488™ Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 488 Antibody generated in donkey detects reactivity to Rabbit IgG.

    Aufreinigung

    Preadsorption: Pre-Adsorbed

    Immunogen

    Rabbit IgG whole molecule

    Labeling Ratio

    6.3
  • Applikationshinweise

    FLISA_Dilution: >1:20,000

    IF_Microscopy_Dilution: >1:5,000

    Western_Blot_Dilution: >1:10,000

    Other: User Optimized

    Kommentare

    Anti-Rabbit IgG (H&L) DyLight 488 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
    Suggested Applications: IF, IHC, Multiplex

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration

    1.0 mg/mL

    Buffer

    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    Haltbarkeit

    12 months
  • Al Amin, Salahuddin, Imai, Hiramatsu: "Characteristics of Enteroendocrine Cells of White Leghorn Chickens, Gallus gallus, Before and After Hatching." in: The journal of poultry science, Vol. 60, pp. 2023029, (2023) (PubMed).

    Salahuddin, Hiramatsu, Kita: "Dietary carbohydrate influences the colocalization pattern of Glucagon-like Peptide-1 with neurotensin in the chicken ileum." in: Domestic animal endocrinology, Vol. 79, pp. 106693, (2022) (PubMed).

    Salahuddin, Hiramatsu, Nishimoto, Kita: "Dietary carbohydrate modifies the density of L cells in the chicken ileum." in: The Journal of veterinary medical science, Vol. 84, Issue 2, pp. 265-274, (2022) (PubMed).

    Estrada-Cuzcano, Etard, Delvallée, Stoetzel, Schaefer, Scheidecker, Geoffroy, Schneider, Studer, Mattioli, Chennen, Sigaudy, Plassard, Poch, Piton, Strahle, Muller, Dollfus: "Novel IQCE variations confirm its role in postaxial polydactyly and cause ciliary defect phenotype in zebrafish." in: Human mutation, Vol. 41, Issue 1, pp. 240-254, (2021) (PubMed).

    Golenberg, Squirrell, Bennin, Rindy, Pistono, Eliceiri, Shelef, Kang, Huttenlocher: "Citrullination regulates wound responses and tissue regeneration in zebrafish." in: The Journal of cell biology, Vol. 219, Issue 4, (2020) (PubMed).

    Nishimura, Hiramatsu, Watanabe, Kita: "Glucagon-like peptide-1 is co-localized with neurotensin in the chicken ileum." in: Cell and tissue research, Vol. 368, Issue 2, pp. 277-286, (2017) (PubMed).

    Wang, Burton, Behringer, Larina: "In vivo micro-scale tomography of ciliary behavior in the mammalian oviduct." in: Scientific reports, Vol. 5, pp. 13216, (2016) (PubMed).

    Nishino, Sasaki, Chihara, Nagasaki, Torigoe, Kon, Agui: "Distinct distribution of the tensin family in the mouse kidney and small intestine." in: Experimental animals, Vol. 61, Issue 5, pp. 525-32, (2013) (PubMed).

    Scoles, Pflieger, Thai, Hansen, Dansithong, Pulst: "ETS1 regulates the expression of ATXN2." in: Human molecular genetics, Vol. 21, Issue 23, pp. 5048-65, (2013) (PubMed).

  • Target

    IgG

    Substanzklasse

    Antibody

    Hintergrund

    Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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