Ziege anti-Kaninchen IgG Antikörper (DyLight 488) - Preadsorbed

Produktnummer ABIN6699099

Hersteller Produkt- Nr.: 611-141-122

Kurzübersicht für Ziege anti-Kaninchen IgG Antikörper (DyLight 488) - Preadsorbed (ABIN6699099)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 488

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Kaninchen

Wirt: Ziege

Hersteller Produkt- Nr.: 611-141-122

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody DyLight™ 488 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Produktmerkmale: Goat anti-Rabbit IgG Antibody DyLight™488 Conjugation, Goat anti-Rabbit IgG DyLight™ 488 Conjugated Antibody,Anti-Rabbit IgG (H&L) DyLight 488 Antibody generated in goat detects reactivity to Rabbit IgG.

Aufreinigung:

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.  This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.

Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Labeling Ratio: 5.4

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000-1:25,000

Other: User Optimized

Kommentare:

Anti-Rabbit IgG (H&L) DyLight 488 Antibody has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: IF, Multiplex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Iguchi, Carrasco, Xie, Pineda, Malykhina, Wilcox: "Functional constipation induces bladder overactivity associated with upregulations of Htr2 and Trpv2 pathways." in: Scientific reports, Vol. 11, Issue 1, pp. 1149, (2021) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.