Ziege anti-Kaninchen IgG Antikörper (Cy5.5) - Preadsorbed

Produktnummer ABIN6699093

Hersteller Produkt- Nr.: 611-113-122

Kurzübersicht für Ziege anti-Kaninchen IgG Antikörper (Cy5.5) - Preadsorbed (ABIN6699093)

Target: IgG

Klonalität: Polyklonal

Konjugat: Cy5.5

Applikation: ELISA, Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Kaninchen

Wirt: Ziege

Hersteller Produkt- Nr.: 611-113-122

Hersteller: Rockland

Verwendungszweck: Rabbit IgG (H&L) Antibody CY5.5 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins

Produktmerkmale: Goat Anti Rabbit IgG Antibody CY5.5 Conjugation, Goat Anti-Rabbit IgG CY5.5 Conjugated Antibody,Anti-Rabbit IgG Antibody CY5.5 generated in goat detects rabbit IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Rabbit IgG whole molecule

Labeling Ratio: 8.2

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Kommentare:

Anti-Rabbit IgG Antibody CY5.5 has been tested by ELISA, dot blot, and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Yamashita, Ancillotti, Rangel, Fontenele, Figueiredo-Freitas, Possidonio, Soares, Sorenson, Mermelstein, Nogueira: "Balance between S-nitrosylation and denitrosylation modulates myoblast proliferation independently of soluble guanylyl cyclase activation." in: American journal of physiology. Cell physiology, Vol. 313, Issue 1, pp. C11-C26, (2017) (PubMed).

Heffron, Mandell: "Opposing roles of ERK and p38 MAP kinases in FGF2-induced astroglial process extension." in: Molecular and cellular neurosciences, Vol. 28, Issue 4, pp. 779-90, (2005) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rabbit IgG (H&L) is conjugated to CY5.5.