Ziege anti-Human IgG Antikörper (DyLight 680) - Preadsorbed

Produktnummer ABIN6698937

Hersteller Produkt- Nr.: 609-144-123

Kurzübersicht für Ziege anti-Human IgG Antikörper (DyLight 680) - Preadsorbed (ABIN6698937)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 680

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 609-144-123

Hersteller: Rockland

Verwendungszweck: Human IgG (H&L) Antibody DyLight™ 680 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Ch Gt GP Ham Hs Ms Rb Rt & Sh Serum Proteins

Produktmerkmale: Goat Anti Human IgG DyLight 680™ Conjugated Antibody, Goat Anti-Human IgG Antibody DyLight 680™ conjugation,Anti-Human IgG (H&L) DyLight 680 generated in goat detects human Immunoglobulin G (IgG), both heavy and light chains of the antibody molecule are present.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Human IgG whole molecule

Labeling Ratio: 3.0

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

Anti-Human IgG (H&L) DyLight 680 has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Suggested Applications: Microarray, WB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Russo, Unkauf, Meier, Wenzel, Langreder, Schneider, Wiesner, Bischoff, Stadler, Dübel: "In vitro evolution of myc-tag antibodies: in-depth specificity and affinity analysis of Myc1-9E10 and Hyper-Myc." in: Biological chemistry, Vol. 403, Issue 5-6, pp. 479-494, (2022) (PubMed).

Edmiston, Jones, Vu, Ashwood, Van de Water: "Identification of the antigenic epitopes of maternal autoantibodies in autism spectrum disorders." in: Brain, behavior, and immunity, Vol. 69, pp. 399-407, (2019) (PubMed).

Aliprandini, Takata, Lepique, Kalil, Boscardin, Moro: "An oligoclonal combination of human monoclonal antibodies able to neutralize tetanus toxin in vivo." in: Toxicon: X, Vol. 2, pp. 100006, (2019) (PubMed).

Freire, Pol-Fachin, Coêlho, Viana, Magalhães, Cordeiro, Fischer, Loeffler, Jaenisch, Franca, Marques, Lins: "Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses." in: ACS omega, Vol. 2, Issue 7, pp. 3913-3920, (2017) (PubMed).

Mock, Warta, Geisenberger, Bischoff, Schulte, Lamszus, Stadler, Felgenhauer, Schichor, Schwartz, Matschke, Jungk, Ahmadi, Sahm, Capper, Glass, Tonn, Westphal, von Deimling, Unterberg, Bermejo et al.: "Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients. ..." in: Oncotarget, Vol. 6, Issue 15, pp. 13579-90, (2016) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: It is a protein complex composed of four peptide chains - two identical heavy chains and two identical light chains arranged in a Y-shape typical of antibody monomers. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.