Ziege anti-Armenischer Hamster IgG Antikörper (DyLight 649) - Preadsorbed

Produktnummer ABIN6698892

Hersteller Produkt- Nr.: 620-143-440

Kurzübersicht für Ziege anti-Armenischer Hamster IgG Antikörper (DyLight 649) - Preadsorbed (ABIN6698892)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 649

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Syrischer Goldhamster, Armenischer Hamster

Wirt: Ziege

Hersteller Produkt- Nr.: 620-143-440

Hersteller: Rockland

Verwendungszweck: Golden Syrian & Armenian Hamster IgG (H&L) Antibody DyLight™ 649 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Mouse and Rat Serum Proteins

Produktmerkmale: goat anti-Golden Syrian & Armenian Hamster IgG DyLight™649 conjugated antibody, goat anti-Hamster IgG DyLight™ 649 conjugated antibody,Anti-Golden Syrian & Armenian Hamster IgG DyLight Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule

Labeling Ratio: 3.2

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.