Kaninchen anti-Pferd IgG Antikörper (Cy3)

Produktnummer ABIN6698832

Hersteller Produkt- Nr.: 708-404-002

Kurzübersicht für Kaninchen anti-Pferd IgG Antikörper (Cy3) (ABIN6698832)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Cy3

Applikation: Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Pferd

Wirt: Kaninchen

Hersteller Produkt- Nr.: 708-404-002

Hersteller: Rockland

Verwendungszweck: F(ab')2 Horse IgG (H&L) Antibody Cy3 Conjugated

Produktmerkmale: Rabbit F(ab')2 Anti-Horse IgG Antibody CY3 Conjugation, Rabbit F(ab')2 Anti-Horse IgG CY3 Conjugated Antibody, Rabbit Fab2 Anti-Horse IgG CY3 Conjugated Antibody,F(ab')2 Anti-Horse IgG Cy3 Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Immunogen: Horse IgG whole molecule

Labeling Ratio: 5.1

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: 1:10,000 - 1:50,000

Flow_Cytometry_Dilution: 1:500 - 1:2,500

IF_Microscopy_Dilution: 1:1,000 - 1:5,000

Other: FLOW CYTOMETRY 1:500 - 1:2,500

Kommentare:

Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 250 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.