Ziege anti-Huhn IgG Antikörper (DyLight 405) - Preadsorbed

Produktnummer ABIN6698806

Hersteller Produkt- Nr.: 603-146-126

Kurzübersicht für Ziege anti-Huhn IgG Antikörper (DyLight 405) - Preadsorbed (ABIN6698806)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 405

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Huhn

Wirt: Ziege

Hersteller Produkt- Nr.: 603-146-126

Hersteller: Rockland

Verwendungszweck: Chicken IgG (H&L) Antibody DyLight™ 405 Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins

Produktmerkmale: goat anti-Chicken IgG DyLight™405 Conjugated Antibody, goat anti-Chicken IgG Antibody DyLight™405 Conjugation, Chicken Secondary Antibody, goat anti-Chicken IgY DyLight™405,Anti-Chicken IgG DyLight Antibody generated in goat detects chicken IgY.

Aufreinigung: Preadsorption: Pre-Adsorbed

Immunogen: Chicken IgG whole molecule

Labeling Ratio: 2.0

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: >1:10,000

Other: User Optimized

Kommentare:

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation. Conjugated Secondary Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Suggested Applications: IF, Multiplex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store conjugated secondary antibody at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature. Conjugated Secondary Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen

Göttle, Förster, Gruchot, Kremer, Hartung, Perron, Küry: "Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination." in: Glia, Vol. 67, Issue 1, pp. 160-170, (2019) (PubMed).

Göttle, Manousi, Kremer, Reiche, Hartung, Küry: "Teriflunomide promotes oligodendroglial differentiation and myelination." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 76, (2019) (PubMed).

Göttle, Sabo, Heinen, Venables, Torres, Tzekova, Parras, Kremer, Hartung, Cate, Küry: "Oligodendroglial maturation is dependent on intracellular protein shuttling." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 3, pp. 906-19, (2015) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.