Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN619596

Kurzübersicht für Ziege anti-Human IgG (Heavy & Light Chain) Antikörper (Alkaline Phosphatase (AP)) (ABIN619596)

Target: IgG

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Human

Wirt: Ziege

Kreuzreaktivität: Schaf, Maus, Kaninchen

Homologie: human IgG (H&L), F(ab)'2 fragment

Produktmerkmale: Goat anti-human IgG (H&L), F(ab) '2 fragment is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgG (H&L), F(ab) '2 fragment in immunological assays.
F(ab)'2 fragment

Aufreinigung: Affinity purification

Immunogen: purified human IgG (H&L)

Anwendungsinformationen

Applikationshinweise: 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Kommentare:

This antibody reacts with the heavy (gamma) chains on human IgG and light chains on all human immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin human serum proteins and serum from mouse, rabbit of bovine serum proteins based on immunoelectrophoresis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Antigendetails

Target: IgG

Substanzklasse: Antibody