Ziege anti-Human IgE (Chain epsilon) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN619576

Kurzübersicht für Ziege anti-Human IgE (Chain epsilon) Antikörper (Alkaline Phosphatase (AP)) (ABIN619576)

Target: IgE

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Chain epsilon

Reaktivität: Human

Wirt: Ziege

Kreuzreaktivität: Schaf, Maus, Kaninchen

Homologie: human IgE heavy (epsilon chain)

Produktmerkmale: Goat anti-human IgE heavy (epsilon chain) is a secondary antibody conjugated to AP (Alkaline phosphatase) which binds to human IgE heavy (epsilon chain) in immunological assays.

Aufreinigung: Affinity purification

Immunogen: purified human IgE

Anwendungsinformationen

Applikationshinweise: 1: 500 - 2 000 (ELISA) and (WB), 1: 20 -1: 2000 (IHC), 1: 50 - 1: 5000 (ICC)

Kommentare:

No reactivity is observed to light chains or non-IgE human serum proteins based in immunoelectrophoresisno reactivity to bovine, mouse or rabbit serum proteins based on immunoelectrophoresis

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Antigendetails

Target: IgE