Kaninchen anti-Human IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN619410

Kurzübersicht für Kaninchen anti-Human IgG (Heavy & Light Chain) Antikörper (HRP) (ABIN619410)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Human

Wirt: Kaninchen

Produktmerkmale: Rabbit anti-human IgG (H&L) is a secondary antibody conjugated to HRP, which binds to human IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase human IgG (H&L).

Aufreinigung: Affinity purification

Immunogen: Purified human IgG (H&L) CAA10835

Anwendungsinformationen

Applikationshinweise: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handhabung: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Antigendetails

Target: IgG

Substanzklasse: Antibody