Ziege anti-Human IgM (Chain mu) Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN619228

Kurzübersicht für Ziege anti-Human IgM (Chain mu) Antikörper (Alkaline Phosphatase (AP)) (ABIN619228)

Target: IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Chain mu

Reaktivität: Human

Wirt: Ziege

Kreuzreaktivität: Human

Produktmerkmale: Goat anti-human IgM (µ chain), ALP (alkaline phosphatase) conjugated, is a F(ab) '2 fragment of a secondary antibody which binds to human IgM in immunological assays. Antibodies are adsorbed against human IgG or IgA. Antibody solution may contain small amounts of intact IgG.
F(ab)'2 fragment

Aufreinigung: Affinity purification

Reinheit: > 90 % based on SDS-PAGE.

Anwendungsinformationen

Applikationshinweise: 1:500-1:2000 for ELISA/western blot, 1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Each reagent is stable for the period shown on the bottle label if stored as directed.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Antigendetails

Target: IgM

Substanzklasse: Antibody