Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated, is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human, mouse IgG and affinity purified using solid phase rat IgG.
1:20-1: 2000 for immunohistochemistry (IHC), 1:50-1: 5000 for immunocytochemistry (ICC) and flow cytometry.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Handhabung
Product is photosensitive and should be protected from light.
Lagerung
4 °C
Informationen zur Lagerung
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.