Ziege anti-Human IgM (Chain mu) Antikörper (HRP)

Produktnummer ABIN618950

Kurzübersicht für Ziege anti-Human IgM (Chain mu) Antikörper (HRP) (ABIN618950)

Target: IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: HRP

Applikation: Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Chain mu

Reaktivität: Human

Wirt: Ziege

Produktmerkmale: Goat anti-human IgM, µ chain, is a F(ab) '2 fragment, of HRP conjugated secondary antibody which binds to human IgM, µ chain in immunological assays. Antibodies are affinity purified using solid phase human IgM.

Aufreinigung: Affinity purification

Anwendungsinformationen

Applikationshinweise: 1:500-1: 5000 for immunohistochemistry (IHC), 1:10 000-1:50 000 for ELISA/western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handhabung: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Informationen zur Lagerung: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Antigendetails

Target: IgM

Substanzklasse: Antibody