Maus anti-Human kappa Light Chain Antikörper (CF®594)

Produktnummer ABIN6173980

Kurzübersicht für Maus anti-Human kappa Light Chain Antikörper (CF®594) (ABIN6173980)

Target: kappa Light Chain

Klonalität: Monoklonal

Konjugat: CF®594

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: HP6053

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Verwendungszweck: Mouse Monoclonal anti-Human Kappa Light Chain (HP6053), CF594 Conjugate

Produktmerkmale: This MAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human Ig kappa chain

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 0.5-1.0 μg/mL
• Flow Cytometry 0.5-2.0 μg/million cells in 0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

293T, Raji or hPBL cells. Tonsil or Spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: kappa Light Chain

Hintergrund: HCAK1, Ig Kappa Chain C Region, IGKC, Immunoglobulin KM

Molekulargewicht: ~22.5 kDa

Gen-ID: 3514, 449609