Rekombinanter Kaninchen anti-Human IgG Antikörper (CF®568)

Produktnummer ABIN6170854

Kurzübersicht für Rekombinanter Kaninchen anti-Human IgG Antikörper (CF®568) (ABIN6170854)

Target: IgG

Antikörpertyp: Recombinant Antibody

Klonalität: Monoklonal

Konjugat: CF®568

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: IG1707R

Isotyp: IgG kappa

Produktdetails

Reaktivität: Human

Wirt: Kaninchen

Verwendungszweck: Recombinant Rabbit Monoclonal anti-Human IgG (IG1707R), CF568 Conjugate

Produktmerkmale: This antibody recognizes a protein of 75 kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This antibody is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified human Ig Gamma Chain

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

293T, Raji or hPBL cells. Tonsil or Spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: IgG

Substanzklasse: Antibody

Molekulargewicht: 75 kDa

Gen-ID: 510635