Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (DyLight 550)

Produktnummer ABIN612393

Kurzübersicht für Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (DyLight 550) (ABIN612393)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 550

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ziege

Verwendungszweck: Goat anti-Rabbit IgG (H&L) - Affinity Pure, DyLight ®550 Conjugate, min x w/human serum proteins

Spezifität: Based on IEP, this antibody reacts with: • heavy (i³) chains on rabbit IgG • light chains on all rabbit immunoglobulins

Kreuzreaktivität (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin rabbit serum proteins • human serum proteins

Aufreinigung: Affinity purified using solid phase Rabbit IgG

Reinheit: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Rabbit IgG, whole molecule

Anwendungsinformationen

Kommentare:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Konzentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.

Lagerung: 4 °C

Informationen zur Lagerung: Store freeze-dried powder at 2-8 °C.

Haltbarkeit: 4 weeks

Referenzen

Michell, Chen Zp, Tiganis, Stapleton, Katsis, Power, Sim, Kemp: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, (2001) (PubMed).

Dimmeler, Fleming, Fisslthaler, Hermann, Busse, Zeiher: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." in: Nature, Vol. 399, Issue 6736, pp. 601-5, (1999) (PubMed).

Gallis, Corthals, Goodlett, Ueba, Kim, Presnell, Figeys, Harrison, Berk, Aebersold, Corson: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." in: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, (1999) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody