Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (DyLight 350)

Produktnummer ABIN612374

Kurzübersicht für Ziege anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (DyLight 350) (ABIN612374)

Target: IgG

Konjugat: DyLight 350

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ziege

Produktmerkmale: Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 350 Conjugate.
Fluorphore: DyLight 350 (Ex = 353 nm, Em = 432 nm).
Fluor Protein Ratio: Moles DyLight 350 per Mole Antibody.

Aufreinigung: Affinity purified using solid phase human IgG (H&L)

Reinheit: > 95 % based on SDS-PAGE

Immunogen: Purified rabbit IgG, whole molecule

Anwendungsinformationen

Applikationshinweise: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Kommentare:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C

Referenzen

Yuryev, Ono, Goff, Macaluso, Wennogle: "Isoform-specific localization of A-RAF in mitochondria." in: Molecular and cellular biology, Vol. 20, Issue 13, pp. 4870-8, (2000) (PubMed).

Barnier, Papin, Eychène, Lecoq, Calothy: "The mouse B-raf gene encodes multiple protein isoforms with tissue-specific expression." in: The Journal of biological chemistry, Vol. 270, Issue 40, pp. 23381-9, (1995) (PubMed).

Eychène, Dusanter-Fourt, Barnier, Papin, Charon, Gisselbrecht, Calothy: "Expression and activation of B-Raf kinase isoforms in human and murine leukemia cell lines." in: Oncogene, Vol. 10, Issue 6, pp. 1159-65, (1995) (PubMed).

Sithanandam, Kolch, Duh, Rapp: "Complete coding sequence of a human B-raf cDNA and detection of B-raf protein kinase with isozyme specific antibodies." in: Oncogene, Vol. 5, Issue 12, pp. 1775-80, (1991) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody