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Ziege anti-Human Ig (Chain kappa), (Light Chain) Antikörper (DyLight 650)

ELISA, FACS, IF, IM DyLight 650

Produktnummer ABIN612192

Datenblatt (PDF)

Target Alle Ig Produkte

Ig
Bindungsspezifität
89

84

64

32

4

4

2

1

1

1
Chain kappa, Light Chain
Reaktivität
125

68

22

19

9

4

2

1

1

1

1

1

1

1

1

1
Human
Wirt
109

84

36

21

2

1

1
Ziege
Konjugat
55

27

27

21

17

14

11

11

5

5

4

4

4

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1
DyLight 650
Applikation
179

146

100

71

57

26

22

14

12

10

9

6

4

2

2

1

1

1

1

1

1

1

1

1
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)

Spezifität

Based on IEP, this antibody reacts with: kappa (κ) light chains on all human immunoglobulins

Kreuzreaktivität (Details)

Based on IEP, no reactivity is observed to: non-immunoglobulin human serum immunoglobulins and heavy chains on all human immunoglobulins and lambda (λ) light chains on all human immunoglobulins and mouse serum proteins

Produktmerkmale

Concentration: 1.0 mg/mL (E 1 % at 280 nm = 13.0)
Conjugate: DyLight 650 (Ex = 652 nm, Em = 672 nm)

Addtional Info: min x w/mouse serum

Aufreinigung

Affinity purified using solid phase human Kappa (κ) Chain

Reinheit

> 95 % based on SDS-PAGE

Immunogen

Purified human kappa (kappa) light chain
Goat anti-Human Ig (Chain kappa) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin

Goat anti-Human Ig antibody
ELISA, FACS Polyclonal IgG unconjugated F(ab')2 fragment

Goat anti-Human Ig (Heavy & Light Chain) antibody (Biotin)
ELISA, FACS, WB Polyclonal IgG Biotin

Goat anti-Human Ig antibody (Biotin)
ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Human Ig antibody (FITC)
ELISA, FACS Polyclonal IgG FITC F(ab')2 fragment

Goat anti-Human Ig (Heavy & Light Chain) antibody (HRP)
ELISA, WB Polyclonal IgG HRP

Goat anti-Human Ig antibody (PE)
ELISA, FACS Polyclonal IgG PE F(ab')2 fragment

Goat anti-Human Ig (Chain lambda) antibody
ELISA, FACS, IHC, WB Polyclonal IgG unconjugated

Goat anti-Human Ig (Chain lambda) antibody (HRP)
ELISA, IHC, WB Polyclonal IgG HRP

Goat anti-Human Ig (Chain lambda) antibody (Biotin)
ELISA, FACS, IHC, WB Polyclonal IgG Biotin

Applikationshinweise

This Conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

Kommentare

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Rekonstitution

Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Konzentration

1.0 mg/mL

Buffer

10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C

Informationen zur Lagerung

Store freeze-dried powder at 2-8 °C. Product is stable for up to 4 weeks at 2-8°C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20°C.
Target

Ig

Andere Bezeichnung

Immunoglobulin Ig (Ig Produkte)