Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (DyLight 594)

Produktnummer ABIN611806

Kurzübersicht für Esel anti-Kaninchen IgG (Heavy & Light Chain) Antikörper (DyLight 594) (ABIN611806)

Target: IgG

Konjugat: DyLight 594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Esel

Produktmerkmale: Donkey anti-Rabbit IgG (H&L) - Affinity Pure, DyLight 594 Conjugate.
Fluorphore: DyLight 594 (Ex = 593 nm, Em = 618 nm).
Fluor Protein Ratio: Moles DyLight 594 per Mole Antibody.

Aufreinigung: Affinity purified using solid phase rat IgG (H&L)

Reinheit: > 95 % based on SDS-PAGE

Immunogen: Purified Rabbit IgG, whole molecule

Anwendungsinformationen

Applikationshinweise: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Kommentare:

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Lagerung: 4 °C

Referenzen

McLean, Fincham, Frame: "v-Src induces tyrosine phosphorylation of focal adhesion kinase independently of tyrosine 397 and formation of a complex with Src." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23333-9, (2000) (PubMed).

Ruest, Roy, Shi, Mernaugh, Hanks: "Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 11, Issue 1, pp. 41-8, (2000) (PubMed).

Calalb, Zhang, Polte, Hanks: "Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src." in: Biochemical and biophysical research communications, Vol. 228, Issue 3, pp. 662-8, (1997) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody