Esel anti-Ziege IgG (Heavy & Light Chain) Antikörper (DyLight 633)

Produktnummer ABIN611723

Kurzübersicht für Esel anti-Ziege IgG (Heavy & Light Chain) Antikörper (DyLight 633) (ABIN611723)

Target: IgG

Klonalität: Polyklonal

Konjugat: DyLight 633

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ziege

Wirt: Esel

Verwendungszweck: Donkey anti-Goat IgG (H&L) - Affinity Pure, DyLight ®633 Conjugate

Spezifität: Based on IEP, this antibody reacts with: • heavy (i³) chains on goat IgG • light chains on all goat immunoglobulins

Kreuzreaktivität (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin goat serum immunoglobulins

Aufreinigung: Affinity purified using solid phase Goat IgG

Reinheit: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Goat IgG, whole molecule

Anwendungsinformationen

Kommentare:

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.

Lagerung: 4 °C

Informationen zur Lagerung: Store freeze-dried powder at 2-8 °C.

Haltbarkeit: 4 weeks

Referenzen

McLean, Fincham, Frame: "v-Src induces tyrosine phosphorylation of focal adhesion kinase independently of tyrosine 397 and formation of a complex with Src." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23333-9, (2000) (PubMed).

Ruest, Roy, Shi, Mernaugh, Hanks: "Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 11, Issue 1, pp. 41-8, (2000) (PubMed).

Calalb, Zhang, Polte, Hanks: "Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src." in: Biochemical and biophysical research communications, Vol. 228, Issue 3, pp. 662-8, (1997) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody