Kaninchen anti-Affe IgG (Heavy & Light Chain) Antikörper (FITC)

Produktnummer ABIN5706887

Hersteller Produkt- Nr.: 617-402-002

Kurzübersicht für Kaninchen anti-Affe IgG (Heavy & Light Chain) Antikörper (FITC) (ABIN5706887)

Target: IgG

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Affe

Wirt: Kaninchen

Hersteller Produkt- Nr.: 617-402-002

Hersteller: Rockland

Verwendungszweck: Monkey IgG (H&L) Antibody Fluorescein

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Anti-Fluorescein, Monkey IgG and Monkey Serum.

Produktmerkmale: Anti-Monkey IgG (H&L) antibody generated in rabbit detects specifically monkey IgG heavy and light chains. This secondary antibody anti-Monkey is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Monkey IgG coupled to agarose beads.

Immunogen:

Immunogen: Anti-Monkey IgG (H&L) was produced by repeated immunization with monkey IgG in rabbit.

Immunogen Type: Native Protein

Labeling Ratio: 3.5

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Monkey IgG (H&L) FITC conjugated Antibody is suitable for use in immunoelectrophoresis, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. Specific conditions for reactivity and signal detection should be optimized by the end user. Flow Cytometry Dilution: User Optimized Western Blot Dilution: 1:10,000 - 1:60,000 FLISA Dilution: 1:50,000 - 1:100,000 IF Microscopy Dilution: 1:1,000 - 1:5,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. This fluorescence conjugated anti-Monkey IgG (H&L) secondary antibody is ideal for investigators who routinely perform immunofluorescence, flow cytometry, and more general immunoassays. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.