Kurzübersicht für Ziege anti-Human IgA (Free & Bound), (Secretory Component) Antikörper (Biotin) (ABIN568605)
Target
IgA
Klonalität
Polyklonal
Konjugat
Biotin
Applikation
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunodiffusion (ID), Dot Blot (DB)
Isotyp
IgG
Bindungsspezifität
Free & Bound, Secretory Component
Reaktivität
Human
Wirt
Ziege
Produktmerkmale
Molar Ratio: Biotin/IgG aprox. 4.5
Aufreinigung
Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and purification of the IgG fraction by DEAE-Chromatography.
Immunogen
Purified free Human Secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
Restore by adding 1.0 mL of sterile distilled water.
Konzentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Konservierungsmittel
Without preservative
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one week or (in aliquots) at -28 °C for longer.
Target
IgA
Substanzklasse
Antibody
Hintergrund
Secretory component is present in human secretions bound to secretory IgA (sIgA) and in free form. Secretory IgA (sIgA) functions as a dimer or polymer and accounts for almost all specific mucosal antibody activity. A molecule of sIgA is made up of two molecules of IgA, one J chain and one SC (MW 65,000). The dimer IgA is transported into secretions by its binding to the SC on the epithelial cells. Under normal conditions, sIgA contains both subclasses IgA1 and IgA2, since both are capable of binding SC. SC also has an affinity for polymeric IgM.Synonyms: Human Immunoglobulin A