Kaninchen anti-Maus kappa Light Chain Antikörper (Biotin)

Produktnummer ABIN5608184

Hersteller Produkt- Nr.: 610-4610

Kurzübersicht für Kaninchen anti-Maus kappa Light Chain Antikörper (Biotin) (ABIN5608184)

Target: kappa Light Chain

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Kaninchen

Hersteller Produkt- Nr.: 610-4610

Hersteller: Rockland

Verwendungszweck: Mouse (kappa chain) Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum.  Specificity was confirmed by ELISA at less than 1 % against other Mouse heavy or light chain proteins.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse kappa light chain

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse κ (kappa chain) (RABBIT) Antibody has been tested by ELISA and is designed for dot blot or western blotting, ELISA, or other detection assays. Immunohistochemistry Dilution: 1:2,000 - 1:10,000 Western Blot Dilution: 1:10,000 - 1:50,000 ELISA Dilution: 1:600,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: kappa Light Chain

Hintergrund: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse kappa light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG kappa is conjugated to biotin.