Esel anti-Human IgM Antikörper (PE) - Preadsorbed

Produktnummer ABIN5608181

Hersteller Produkt- Nr.: 709-708-031

Kurzübersicht für Esel anti-Human IgM Antikörper (PE) - Preadsorbed (ABIN5608181)

Target: IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: PE

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Esel

Hersteller Produkt- Nr.: 709-708-031

Hersteller: Rockland

Verwendungszweck: F(ab')2 Human IgM Fc5u Antibody Phycoerythrin Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv & Hs Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Human IgM, Human IgM Fc5μ and Human Serum.  No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), Bovine or Horse Serum Proteins, or other human heavy or light chain proteins.

Produktmerkmale: F(ab')2 Anti-Human IgG (H&L) Biotin Antibody generated in donkey detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Human IgM Fc5mu  fragment

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications. Flow Cytometry Dilution: 1:100 - 1:250 IF Microscopy Dilution: 1:100 - 1:250 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 0.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.

Haltbarkeit: 6 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.