Ziege anti-Ratte IgA, IgG, IgM Antikörper (TRITC)

Produktnummer ABIN5608162

Hersteller Produkt- Nr.: 612-100-130

Produktdetails

Target: IgA, IgG, IgM

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: TRITC

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA

Hersteller Produkt- Nr.: 612-100-130

Hersteller: Rockland

Verwendungszweck: Rat IgG IgA IgM (H&L) Antibody Rhodamine Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  This reagent is suitable for the detection of all Rat immunoglobulin isotypes and chain combinations.

Produktmerkmale: Anti-Rat IgG (H&L) rhodamine Antibody generated in goat detects reactivity to Rat IgG.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Rat IgG, IgA and IgM whole molecules

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer: Optional[Buffer]: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG,IgA,IgM (IgA, IgG, IgM Produkte)

Substanzklasse: Antibody

Hintergrund: Anti-Rat IgG IgA IgM (H&L) Antibody generated in goat detects rat (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgG IgA IgM Antibody is conjugated to rhodamine.