Ziege anti-Human IgA, IgG, IgM Antikörper (TRITC) - Preadsorbed

Produktnummer ABIN5608152

Hersteller Produkt- Nr.: 709-100-130

Kurzübersicht für Ziege anti-Human IgA, IgG, IgM Antikörper (TRITC) - Preadsorbed (ABIN5608152)

Target: IgA, IgG, IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: TRITC

Applikation: Flow Cytometry (FACS), Fluorescence Microscopy (FM), FLISA

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 709-100-130

Hersteller: Rockland

Verwendungszweck: F(ab')2 Human IgG IgA IgM (H&L) Antibody Rhodamine Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Mouse Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum.  No reaction was observed against anti-Goat IgG F(c), anti-Pepsin or Mouse Serum Proteins.  This product is suitable for the detection of all Human immunoglobulin classes, isotypes and chain combinations.

Produktmerkmale: F(ab')2 Anti-Horse IgG Texas Red Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment.

Aufreinigung: F(ab')2 Anti-HUMAN IgG IgA IgM (H&L) (GOAT) Antibody was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Human IgG, IgA, and IgM whole molecules

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Human IgG IgA IgM (H&L) Rhodamine Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG,IgA,IgM

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Human IgG IgA IgM (H&L) Rhodamine Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsonize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.