Kaninchen anti-Maus kappa Light Chain Antikörper

Produktnummer ABIN5608150

Hersteller Produkt- Nr.: 610-4110

Kurzübersicht für Kaninchen anti-Maus kappa Light Chain Antikörper (ABIN5608150)

Target: kappa Light Chain

Klonalität: Polyklonal

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Maus

Wirt: Kaninchen

Hersteller Produkt- Nr.: 610-4110

Hersteller: Rockland

Verwendungszweck: Mouse kappa Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse IgG and Mouse Serum.   Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes.

Produktmerkmale: Anti-Mouse IgM mu heavy chain antibody generated in rabbit detects specifically Mouse IgM mu heavy chain.

Aufreinigung: Anti-MOUSE kappa (kappa chain) (RABBIT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Mouse kappa light chain

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-MOUSE kappa (kappa chain) (RABBIT) Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:5,000 - 1:50,000 ELISA Dilution: 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: kappa Light Chain

Hintergrund: Anti-Mouse κ (kappa chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.