Ziege anti-Human kappa Light Chain Antikörper

Produktnummer ABIN5608140

Hersteller Produkt- Nr.: 609-1110

Kurzübersicht für Ziege anti-Human kappa Light Chain Antikörper (ABIN5608140)

Target: kappa Light Chain

Klonalität: Polyklonal

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 609-1110

Hersteller: Rockland

Verwendungszweck: Human (kappa chain) Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Specificity for kappa chain was confirmed by ELISA minimal cross reactivity against other human heavy or lambda light chain isotypes.

Produktmerkmale: Anti-Human IgM (mu heavy chain) generated in goat detects specifically Human IgM mu heavy chain.

Aufreinigung: Human κ (kappa chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Human kappa light chain

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human κ antibody has been tested by ELISA and western blot and is suitable for immunohistochemistry and immunoassays where specificity to the immunoglobulin kappa subunit is desired. Optimal concentrations in immunoassays should be determined by the researcher. Immunohistochemistry Dilution: 1:500 - 1:2,000 Western Blot Dilution: 1:1,000 - 1:10,000 ELISA Dilution: 1:10,000 - 1:20,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: kappa Light Chain

Hintergrund: The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain.