Ziege anti-Ratte IgG (F(ab')2 Region) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN5608115

Hersteller Produkt- Nr.: 712-1231

Kurzübersicht für Ziege anti-Ratte IgG (F(ab')2 Region) Antikörper (FITC) - Preadsorbed (ABIN5608115)

Target: IgG

Fragment: F(ab')2 fragment

Konjugat: FITC

Applikation: Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Ratte

Wirt: Ziege

Hersteller Produkt- Nr.: 712-1231

Hersteller: Rockland

Verwendungszweck: F(ab')2 Rat IgG F(ab')2 Antibody Fluorescein Conjugated Pre-Adsorbed

Kreuzreaktivität (Details): Minimal crossreactivity against Bv Hs & Hu Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rat IgG, Rat IgG F(ab')2 and Rat Serum.  No reaction was observed against Rat IgG F(c), anti-Pepsin, anti-Goat IgG F(c) or Bovine, Horse and Human Serum Proteins.

Produktmerkmale: F(ab')2 Anti-Rat IgG F(c) Fluorescein Antibody was generated in goat and detects specifically Rat IgG F(c). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Immunogen: Optional[Immunogen]: Rat IgG F(ab')2 fragment

Anwendungsinformationen

Applikationshinweise: Application Note: F(ab')2 Anti-Rat IgG F(ab')2 Fluorescein Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:50 - 1:200 FLISA Dilution: 1:50 - 1:200 IF Microscopy Dilution: 1:50 - 1:200 Other: FLOW CYTOMETRY 1:500 - 1:2,500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: F(ab')2 Anti-Rat IgG F(ab')2 Fluorescein Antibody generated in goat detects Rat F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.