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Ziege anti-Human kappa Light Chain Antikörper (FITC)

Rockland 709-1210 FACS, WB, ELISA, FM, DB, FLISA Polyclonal IgG FITC F(ab')2 fragment
Rockland
Produktnummer ABIN5608103
Hersteller Produkt- Nr.: 709-1210
  • Target Alle kappa Light Chain Produkte
    kappa Light Chain
    Fragment
    F(ab')2 fragment
    Reaktivität
    • 43
    • 19
    • 2
    • 2
    • 1
    • 1
    Human
    Wirt
    • 21
    • 19
    • 15
    • 4
    • 3
    • 3
    Ziege
    Klonalität
    • 42
    • 23
    Polyklonal
    Konjugat
    • 16
    • 11
    • 8
    • 7
    • 7
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Applikation
    • 40
    • 34
    • 26
    • 19
    • 16
    • 14
    • 9
    • 9
    • 8
    • 8
    • 8
    • 6
    • 5
    • 1
    Flow Cytometry (FACS), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB), FLISA
    Hersteller Produkt- Nr.
    709-1210
    Hersteller
    Rockland
    Verwendungszweck
    F(ab')2 Human (kappa chain) Antibody Fluorescein Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.
    Produktmerkmale
    F(ab')2 Anti-Human IgA Fluorescein Antibody generated in goat detects immunoglobulin A (alpha chain) from human. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    Aufreinigung
    F(ab')2 HUMAN kappa Antibody Fluorescein Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
    Immunogen
    Optional[Immunogen]: Human kappa light chain
    Isotyp
    IgG
  • Applikationshinweise
    Application Note: F(ab')2 Human kappa Fluorescein Conjugated Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry Dilution: 1:500 - 1:2,500 FLISA Dilution: 1:10,000 - 1:50,000 IF Microscopy Dilution: 1:1,000 - 1:5,000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    , Preservative:0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    kappa Light Chain
    Abstract
    kappa Light Chain Produkte
    Hintergrund
    F(ab')2 HUMAN kappa Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
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