Ziege anti-Human kappa Light Chain Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN5608087

Hersteller Produkt- Nr.: 609-1510

Kurzübersicht für Ziege anti-Human kappa Light Chain Antikörper (Alkaline Phosphatase (AP)) (ABIN5608087)

Target: kappa Light Chain

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Isotyp: IgG

Produktdetails

Reaktivität: Human

Wirt: Ziege

Hersteller Produkt- Nr.: 609-1510

Hersteller: Rockland

Verwendungszweck: Human (kappa chain) Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Goat Serum.  Solid phase adsorption(s) against a panel of Human IgG lambda, IgM lambda and IgA lambda proteins was performed.  Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes.

Produktmerkmale: Anti-Human IgM (mu heavy chain) Alkaline Phosphatase generated in goat detects specifically Human IgM mu heavy chain.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Human kappa light chain

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Human kappa chain Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: kappa Light Chain

Hintergrund: The anti-Human κ (kappa chain) Antibody detects the kappa chain subunit. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). In humans the light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Human IgG kappa is conjugated to alkaline phosphatase.