Ziege anti-Frettchen IgM Antikörper (Alkaline Phosphatase (AP))

Produktnummer ABIN5608086

Hersteller Produkt- Nr.: 618-105-007

Kurzübersicht für Ziege anti-Frettchen IgM Antikörper (Alkaline Phosphatase (AP)) (ABIN5608086)

Target: IgM

Klonalität: Polyklonal

Konjugat: Alkaline Phosphatase (AP)

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Frettchen

Wirt: Ziege

Hersteller Produkt- Nr.: 618-105-007

Hersteller: Rockland

Verwendungszweck: Ferret IgM (mu chain) Antibody Alkaline Phosphatase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Ferret IgM and Ferret Serum.  No reaction was observed against other ferret heavy or light chain proteins.

Produktmerkmale: Anti-Ferret IgA Alkaline Phosphatase Antibody generated in goat detects immunoglobulin A from ferret. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA).

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Ferret IgM whole molecule

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Ferret IgM Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:200 - 1:1,000 Western Blot Dilution: 1:500 - 1:2,500 ELISA Dilution: 1:1,000 - 1:2,500 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.1 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Haltbarkeit: 12 months

Antigendetails

Target: IgM

Substanzklasse: Antibody

Hintergrund: Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Ferret IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.