Ziege anti-Human lambda Light Chain (Lambda-IgLC) Antikörper

Produktnummer ABIN5608051

Hersteller Produkt- Nr.: 709-1111

Produktdetails

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Fragment: F(ab')2 fragment

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Hersteller Produkt- Nr.: 709-1111

Hersteller: Rockland

Verwendungszweck: F(ab')2 Human (lambda chain) Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Human IgG and Human Serum.  No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).  Specificity was confirmed by ELISA at less than 1 % cross reactivity against other human heavy or light chain isotypes.

Produktmerkmale: Anti-Human IgG (kappa chain) antibody generated in goat detects specifically human IgG (kappa chain). This secondary antibody anti-Human is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Sterilität: Sterile filtered

Immunogen: Optional[Immunogen]: Human lambda light chain

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:6,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to opening.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. 

Haltbarkeit: 12 months

Antigendetails

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Andere Bezeichnung: lambda light chain (Lambda-IgLC Produkte)

Hintergrund: Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.