Ziege anti-Frettchen IgG Antikörper (Biotin)

Produktnummer ABIN5608045

Hersteller Produkt- Nr.: 618-106-012

Kurzübersicht für Ziege anti-Frettchen IgG Antikörper (Biotin) (ABIN5608045)

Target: IgG

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Reaktivität: Frettchen

Wirt: Ziege

Hersteller Produkt- Nr.: 618-106-012

Hersteller: Rockland

Verwendungszweck: Ferret IgG (gamma chain) Antibody Biotin Conjugated

Kreuzreaktivität (Details): Anti-FERRET IgG (gamma chain) (GOAT) Antibody assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgG and Ferret Serum.  No reaction was observed against Ferret IgA or Ferret IgM.

Produktmerkmale: Anti-Ferret IgM antibody specifically detects ferret IgM.

Aufreinigung: Anti-FERRET IgG (gamma chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Ferret IgG gamma heavy chain

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-FERRET IgG (gamma chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Jackson-Thompson, Kim, Jaiswal, Scott, Jones, Morris, Fite, Laurie, Hoy, Dardzinski, Mitre: "Brugia malayi infection in ferrets - A small mammal model of lymphatic filariasis." in: PLoS neglected tropical diseases, Vol. 12, Issue 3, pp. e0006334, (2018) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.