Ziege anti-Ratte IgG2c Antikörper - Preadsorbed

Produktnummer ABIN458753

Kurzübersicht für Ziege anti-Ratte IgG2c Antikörper - Preadsorbed (ABIN458753)

Target: IgG2c

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Isotyp: IgG

Produktdetails

Reaktivität: Ratte

Wirt: Ziege

Spezifität: The reactivity of the antiserum is directed to the subclass IgG2c as tested against rat sera and a panel of purified homogenous immunoglobulins.

Aufreinigung: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Pools of purified homogenous IgG2c isolated from pooled rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: As unlabelled primary or secondary reagent for indirect detection of IgG2c at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Typical working dilutions in histochemistry are usually between 1:100 and 1:500, in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Konzentration: 10 mg/mL

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and p referably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG2c

Substanzklasse: Antibody