Ziege anti-Ratte IgG2ab, Subclass Specific (Subclass specific) Antikörper - Preadsorbed

Produktnummer ABIN458751

Kurzübersicht für Ziege anti-Ratte IgG2ab, Subclass Specific (Subclass specific) Antikörper - Preadsorbed (ABIN458751)

Target: IgG2ab (IgG2ab, Subclass Specific)

Klonalität: Polyklonal

Applikation: ELISA, Dot Blot (DB), Western Blotting (WB), Indirect Immunofluorescence Assay (IFA)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Subclass specific

Reaktivität: Ratte

Wirt: Ziege

Verwendungszweck: Goat anti Rat IgG2ab (subclass specific)

Spezifität: IgG fraction of polyclonal Goat antiSerum to Rat IgG2ab, subclass specific

Kreuzreaktivität (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested by immunoelectrophoresis and double radial immunodiffusion against rat sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, gM and IgA or any non-Ig protein in rat serum. As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Pools of purified homogenous IgG2a and IgG2b isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized IgG (7S) is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Antigendetails

Target: IgG2ab (IgG2ab, Subclass Specific)

Andere Bezeichnung: IgG2ab