Kaninchen anti-Ratte IgG2c Antikörper

Produktnummer ABIN458720

Kurzübersicht für Kaninchen anti-Ratte IgG2c Antikörper (ABIN458720)

Target: IgG2c

Klonalität: Polyklonal

Applikation: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Isotyp: IgG

Produktdetails

Reaktivität: Ratte

Wirt: Kaninchen

Spezifität: The reactivity of the antiserum is directed to the Fc portion of the subclass IgG2c.

Produktmerkmale: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal rabbit serum. Antibody titre: Precipitin titre not less then 1:32 when tested against pooled normal rat serum in agar block immunodiff

Aufreinigung: Delipidated, heat inactivated, lyophilized whole antiserum.
Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Immunogen: Pools of purified homogenous IgG2c isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2c in rat serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgG2c from serum or plasma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: No foreign proteins added.

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG2c

Substanzklasse: Antibody