Kaninchen anti-Ratte IgA, IgG, IgM (Heavy & Light Chain) Antikörper

Produktnummer ABIN458715

Kurzübersicht für Kaninchen anti-Ratte IgA, IgG, IgM (Heavy & Light Chain) Antikörper (ABIN458715)

Target: IgA, IgG, IgM

Klonalität: Polyklonal

Applikation: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ratte

Wirt: Kaninchen

Verwendungszweck: Rabbit anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains)

Spezifität: Precipitating polyclonal Rabbit antiSerum to Rat immunoglobulins, IgG, IgA and IgM, heavy and light chains.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal rat serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.

Aufreinigung: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Purified polyclonal IgG, including all subclasses and pools of homogenous IgA and IgM isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit s

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG + IgA + IgM

Substanzklasse: Antibody