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Ziege anti-Ratte IgG2c Antikörper (FITC)

IF Polyclonal IgG FITC
Produktnummer ABIN458703

Kurzübersicht für Ziege anti-Ratte IgG2c Antikörper (FITC) (ABIN458703)

Target

IgG2c

Klonalität

  • 26
  • 14
Polyklonal

Konjugat

  • 13
  • 6
  • 6
  • 5
  • 5
  • 2
  • 1
  • 1
  • 1
FITC

Applikation

  • 33
  • 15
  • 13
  • 11
  • 10
  • 8
  • 3
  • 2
  • 2
  • 2
  • 1
Immunofluorescence (IF)

Isotyp

IgG
  • Reaktivität

    • 20
    • 20
    • 1
    Ratte

    Wirt

    • 24
    • 10
    • 4
    • 2
    Ziege

    Spezifität

    The reactivity of the antiserum is directed to the subclass IgG2c as tested against rat sera and a panel of purified homogenous immunoglobulins.

    Produktmerkmale

    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to rat IgG2c, subclass specific
    Fluorochrome/IgG protein molar ratio (F/P) is approximately 4.5.

    Aufreinigung

    Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.

    Immunogen

    Pools of highly purified homogenous IgG2c isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Applikationshinweise

    To identify and measure IgG2c, antigen or antibody, at the cellular and subcellular level by immuno-fluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.

    Kommentare

    Excitation/Emission wavelength: 494 nm/514 nm

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

    Konzentration

    10 mg/mL

    Buffer

    Fluorochrome-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).

    Konservierungsmittel

    Without preservative

    Handhabung

    Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

    Lagerung

    4 °C/-20 °C

    Informationen zur Lagerung

    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Target

    IgG2c

    Substanzklasse

    Antibody
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