Ziege anti-Ratte IgE (Fc Region) Antikörper

Produktnummer ABIN458642

Produktdetails

Target: IgE

Bindungsspezifität: Fc Region

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Applikation: Immunoprecipitation (IP)

Spezifität: Precipitating polyclonal Goat antiSerum to Rat IgE, Fc specific

Kreuzreaktivität (Details): The antiSerum does not cross-react with any other component of the Rat immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, because of the very low level of IgE in most species.

Produktmerkmale: The reactivity of the antiserum is restricted to the Fc part of the IgE molecule, which represents strict isotype (class) specificity. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the mmunogen a single precipitin line is obtained which shows a reaction of full identity with the precipitin lines obtained against rat serum and ascites containing monoclonal IgE. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgE in rat serum or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of rat IgE from serum or plasma. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude the use of the antiserum in more sensitive techniques if proper controls are included.

Aufreinigung: Delipidated, heat inactivated, stable whole serum

Sterilität: Heat-inactivated

Immunogen: Highly purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added. Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.

Konservierungsmittel: Without preservative

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Antigendetails

Target: IgE

Abstract: IgE Produkte