Ziege anti-Kaninchen IgA, IgG, IgM (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN458607

Kurzübersicht für Ziege anti-Kaninchen IgA, IgG, IgM (Heavy & Light Chain) Antikörper (HRP) (ABIN458607)

Target: IgA, IgG, IgM

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Dot Blot (DB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotyp: IgG

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Kaninchen

Wirt: Ziege

Verwendungszweck: Goat anti Rabbit IgG IgA IgM (heavy and light chains), conjugated with Horseradish peroxidase

Spezifität: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Rabbit immunoglobulins IgG, IgA and IgM, heavy and light chains.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is directed to the Fc and Fab subunits of the major Immunoglobulin isotypes. It does not react with any non-Ig protein in rabbit serum, as tested by immuno immunoelectrophoresis and double radial immunodiffusion In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG, antigen or antibody, of appropriately treated cell and tissue substrates at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA) to identify and measure a specific IgG in rabbit serum or other body fluid. In electron microscopy, since the complex between the conjugated antibody and the antigen also has electron-dense properties

Aufreinigung: Purified IgG fraction from antiserum

Immunogen: Purified immunoglobulin fractions isolated from pooled rabbit serum. Freund's complete adjuvant is ed in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Antigendetails

Target: IgA, IgG, IgM

Andere Bezeichnung: IgG + IgA + IgM

Substanzklasse: Antibody