Kaninchen anti-Taube IgG (Heavy & Light Chain) Antikörper

Produktnummer ABIN458578

Kurzübersicht für Kaninchen anti-Taube IgG (Heavy & Light Chain) Antikörper (ABIN458578)

Target: IgG

Klonalität: Polyklonal

Applikation: Immunoprecipitation (IP)

Produktdetails

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Taube

Wirt: Kaninchen

Spezifität: Precipitating polyclonal Rabbit antiSerum to pigeon IgG, heavy and light chains.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. The degree of crossreactivity depends in addition to the phylogenetic relationship also on the concentRation of the reactants and the sensitivity of the assay format. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a reaction with Chicken, Duck and Turkey has been observed

Produktmerkmale: The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in pigeon serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal pigeon serum, to prepare an immunoadsorbent for the production of Ig-free pigeon serum, plasma or non- Ig preparations.

Aufreinigung: Delipidated, heat inactivated, stable whole serum

Sterilität: Heat-inactivated

Immunogen: Purified normal IgG isolated from pooled pigeon serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum.

Konservierungsmittel: Without preservative

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product

Referenzen

Stenzel, Dziewulska, Tykałowski, Koncicki: "The Clinical Infection with Pigeon Circovirus (PiCV) Leads to Lymphocyte B Apoptosis But Has No Effect on Lymphocyte T Subpopulation." in: Pathogens (Basel, Switzerland), Vol. 9, Issue 8, (2020) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody