Schaf anti-Maus Ig lambda (Free & Bound) Antikörper (TRITC)

Produktnummer ABIN458492

Produktdetails

Target: Ig lambda

Bindungsspezifität: Free & Bound

Reaktivität: Maus

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: TRITC

Applikation: ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Sheep antiSerum to free and bound Mouse Ig lambda light chain.

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Produktmerkmale: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda chain. A reaction is obtained with intact polyclonal or monoclonal immunoglobulins of the lambda type, as well as free lambda light chains. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Clone: Polyclonal

Aufreinigung: Purified

Immunogen: A pool of purified Bence Jones lambda proteins isolated from mouse urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Fluorochrome coupled purified sheep IgG lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Fluorochrome coupled purified sheep IgG lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Antigendetails

Target: Ig lambda

Abstract: Ig lambda Produkte