Ziege anti-Maus IgE (Fc Region) Antikörper (TRITC)

Produktnummer ABIN458384

Produktdetails

Target: IgE

Bindungsspezifität: Fc Region

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: TRITC

Applikation: ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgE, Fc specific

Kreuzreaktivität (Details): This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this conjugate has been passed over appropriate immuno-adsorbents to remove antibodies cross-reacting with Mouse immunoglobulins. This renders it specific for use in test systems containing material of Mouse origin (e.g. Mouse tissue/Mouse monoclonal antibody to a Mouse tissue constituent/anti Mouse Ig isotype-specific immunoconjugate.

Produktmerkmale: The reactivity of the antiserum is directed to the Fc subunit of the IgE molecule, which expresses strict (class) specificity. In immunoelectrophoresis and radial in immunodiffusion using various antiserum concentrations against serum of mice belonging to different allotypic groups, a single precipitin line has been obtained which shows a reaction of identity with the precipitin lines obtained with the purified IgE used as immunogens. It does not react with IgG including all subclasses, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. The antiserum also react with membrane-bound IgE in peripheral blood cells of different mouse strains as tested by immunofluorescence microscopy. In immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Aufreinigung: Purified

Immunogen: Pools of purified homogenous IgE isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: TRITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Konservierungsmittel: Without preservative

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Antigendetails

Target: IgE

Abstract: IgE Produkte