Schaf anti-Human IgA (Dimer) Antikörper - Preadsorbed

Produktnummer ABIN458161

Produktdetails

Target: IgA

Bindungsspezifität: Dimer

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Applikation: Immunodiffusion (ID), Immunoelectrophoresis (IEP)

Spezifität: The reactivity of the antiserum is restricted to the Fc part of the IgA molecule and to surface and hidden determinants of the secretory component as tested in immunoelectrophoresis and radial immunodiffusion. In immunoelectrophoresis using various antiserum concentrations against normal human milk two precipitin lines may be obtained, one representing IgA and the other the free secretory component. Against normal human serum only IgA is precipitated. No precipitation reaction is obtained with purified IgG, IgM, and IgG/Fab fragments

Kreuzreaktivität (Details): The antiserum does not cross-react with any other component of the human Ig system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. of this antiserum has not been tested in detail.

Produktmerkmale: Precipitating polyclonal sheep antiserum to human secretory IgA
Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added. Antibody titre: Precipitin titre 1:64 when tested against pooled normal human serum in agar-block immuno-diffusion titra
Delipidated, heat inactivated, lyophilized, stable whole antiserum

Aufreinigung: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens

Immunogen: Highly purified secretory IgA isolated from pooled human milk. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgA in human serum, milk or other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of human IgA from serum or exocrine secretions. Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms as secretory IgA. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. This does not exclude such use if proper controls are include.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not ref rozen, and preferably used the same day.

Antigendetails

Target: IgA

Abstract: IgA Produkte

Substanzklasse: Antibody