Ziege anti-Human IgM (Fc Region) Antikörper (FITC) - Preadsorbed

Produktnummer ABIN458075

Kurzübersicht für Ziege anti-Human IgM (Fc Region) Antikörper (FITC) - Preadsorbed (ABIN458075)

Target: IgM

Fragment: F(ab')2 fragment

Klonalität: Polyklonal

Konjugat: FITC

Applikation: Immunofluorescence (IF)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fc Region

Reaktivität: Human

Wirt: Ziege

Spezifität: The antiserum is reacting with to the Fc subunit of IgM.

Produktmerkmale: Fluorescein isothiocyanate-conjugated F(ab')2 fragments IgG antibodies of polyclonal goat antiserum to human IgM, Fc specific
Fluorochrome/IgG protein molar ratio (F/P) is approximately 1.6.

Aufreinigung: Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.

Immunogen: Purified IgM prepared from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgM. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2

Konservierungsmittel: Without preservative

Handhabung: Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgM

Substanzklasse: Antibody