Ziege anti-Human IgG (Fab Region) Antikörper

Produktnummer ABIN457951

Kurzübersicht für Ziege anti-Human IgG (Fab Region) Antikörper (ABIN457951)

Target: IgG

Klonalität: Polyklonal

Applikation: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Isotyp: IgG

Produktdetails

Bindungsspezifität: Fab Region

Reaktivität: Human

Wirt: Ziege

Verwendungszweck: Goat anti Human IgG Fab

Spezifität: Precipitating polyclonal Goat antiSerum to Human Fab of IgG

Kreuzreaktivität: Affe

Keine Kreuzreaktivität: Cat, Huhn, Rind (Kuh), Hund, Hamster, Pferd, Maus, Schwein, Kaninchen, Ratte, Schaf, Pute

Kreuzreaktivität (Details): Inter-species cross-reactivity is a normal feature of antibodies to Serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine - Dog - Horse - pigeon - Sheep - Cat - Guinea Pig - Monkey ++ Rabbit - Swine - Chicken - Hamster - Mouse - Rat - Turkey - Three Monkey sera were tested. A reaction with Rhesus Monkey, Cynomolgus and Baboon have been found.

Produktmerkmale: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.

Aufreinigung: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Purified Fab of normal human IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Anwendungsinformationen

Applikationshinweise: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum 0.1 % sodium azide. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Antigendetails

Target: IgG

Substanzklasse: Antibody