Maus anti-Human IgG Antikörper

Produktnummer ABIN457927

Kurzübersicht für Maus anti-Human IgG Antikörper (ABIN457927)

Target: IgG

Klonalität: Monoklonal

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Klon: NI 335 and NI 343

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immuno-peroxidase staining of cytoplasmic Ig. Monoclonal mouse antiserum to human IgG, isotype specific

Aufreinigung: Delipidated, heat inactivated, lyophilized, stable whole ascites

Immunogen: Highly purified IgG isolated from pooled human serum

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Konzentration: 1.0 mg/mL

Buffer: No foreign proteins added.

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG

Substanzklasse: Antibody