Maus anti-Human IgG (Fc Region) Antikörper
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- Target Alle IgG Produkte
- IgG
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Bindungsspezifität
- Fc Region
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Applikation
- ELISA, Western Blotting (WB), Immunofluorescence (IF)
- Spezifität
- The reactivity of the antiserum is restricted to isotype specific determinants on the Fc part of the IgG molecule. The antiserum reacts with the 4 subclasses of IgG, as tested in indirect binding enzyme immunoassay, immunoblotting and indirect immuno-peroxidase staining of cytoplasmic Ig. Monoclonal mouse antiserum to human IgG, isotype specific
- Aufreinigung
- Delipidated, heat inactivated, lyophilized, stable whole ascites
- Immunogen
- Highly purified IgG isolated from pooled human serum
- Klon
- NI 335 and NI 343
- Isotyp
- IgG1 kappa
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Mouse anti-Human IgG (Fab Region) antibody
ELISA, WB, FACS Monoclonal 4A11 IgG2b unconjugated
Mouse anti-Human IgG (Fc Region) antibody (HRP)ELISA, IHC, WB Monoclonal JDC-10 IgG1 HRP
Goat anti-Human IgG antibody (PE)ELISA, FACS Polyclonal IgG PE F(ab')2 fragment
Mouse anti-Human IgG (Fc Region) antibody (APC)ELISA, FACS Monoclonal H2 IgG2b APC
Goat anti-Human IgG (Heavy & Light Chain) antibodyELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgG (Fc Region) antibody (Biotin)ELISA, FLISA Polyclonal IgG Biotin
Goat anti-Human IgG (Heavy Chain) antibody (TRITC)ELISA, FACS Polyclonal IgG TRITC
Goat anti-Human IgG (Heavy Chain) antibody (Texas Red (TR))ELISA, FACS Polyclonal IgG Texas Red (TR)
Goat anti-Human IgG antibody (Biotin)ELISA, FACS Polyclonal IgG Biotin F(ab')2 fragment
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- Applikationshinweise
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To identify the presence of IgG in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immuno-peroxidase and immunofluorescence staining and immunoblotting of cytoplasmic IgG.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.
- Konzentration
- 1.0 mg/mL
- Buffer
- No foreign proteins added.
- Handhabung
- Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
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- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
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